TB-500 (TB4) Kit (20mg – 100mg)

TB-500 (TB4) Kit

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Peptide Partners Manufacturer Id: SH07

Batch Id: TB20250614

Research Studies

(for educational purposes only)

Study 1: Simultaneous quantification of TB-500 and its metabolites in in-vitro experiments and rats by UHPLC-Q-Exactive orbitrap MS/MS and their screening by wound healing activities in-vitro

Authors: Khandoker Asiqur Rahaman, Anca Raluca Muresan, Hophil Min, Junghyun Son, Hyung-Seop Han, Min-Jung Kang, Oh-Seung Kwon

Source: https://www.sciencedirect.com/science/article/pii/S1570023224000412

Scientific Findings

This study developed and validated a simultaneous analytical method for TB-500 and its metabolites using UHPLC-Q-Exactive orbitrap MS. The metabolism of TB-500 was investigated in human serum, various in-vitro enzyme systems, and in urine from rats. The biological activities of the parent compound and its metabolites were assessed via cytotoxicity and wound healing experiments in fibroblasts. The results indicated that Ac-LK was the primary metabolite in rats, while Ac-LKK was a long-term metabolite. No cytotoxicity was observed for the parent compound or its metabolites. Notably, only the metabolite Ac-LKKTE demonstrated significant wound healing activity compared to the control, suggesting that the reported therapeutic effects of TB-500 may be attributable to this metabolite rather than the parent form.

Plain English Interpretation

Scientists have developed a new method to measure TB-500 and its breakdown products in the body. TB-500 is a substance that is thought to help with healing. In this study, they looked at how TB-500 is broken down in human blood and in rats. They found that one of the breakdown products, called Ac-LKKTE, is actually what helps wounds to heal, not TB-500 itself. This is an important discovery because it could lead to new and better treatments for wounds in the future.

Study 2: Investigation of in vitro/ex vivo TB-500 metabolism, synthesis of relevant metabolites and detection limits in urine and plasma

Authors: P. Van Eenoo

Source: https://www.wada-ama.org/en/resources/scientific-research/investigation-vitroex-vivo-tb-500-metabolism-synthesis-relevant

Scientific Findings

This project aimed to characterize the human metabolism of TB-500 using in vitro (human liver microsomes and S9 fraction) and ex vivo (human plasma/serum) models. The study found that TB-500 undergoes serial cleavage at the C-terminus, while the N-terminal acetylation provides protection. The researchers synthesized three metabolites, TB-500 M(1-2), TB-500 M(1-3), and TB-500 M(1-5), and a deuterated internal standard (TB-500-d3). The limits of detection (LODs) for the synthesized metabolites were determined and implemented into a screening method for detecting peptides in urine, with LODs of 500 pg/mL for TB-500 M(1-2), 100 pg/mL for TB-500 M(1-3), and 50 pg/mL for TB-500 M(1-5).

Plain English Interpretation

Researchers studied how the human body breaks down TB-500, a substance sometimes used to enhance athletic performance. They used laboratory models of the human liver and blood to see what happens to TB-500. They found that the substance is broken down in a specific way, and they were able to create some of these breakdown products in the lab. This information was then used to develop a more sensitive test to detect TB-500 in urine, which is important for anti-doping efforts in sports.

Study 3: Utilizing Developmentally Essential Secreted Peptides Such as Thymosin Beta-4 to Remind the Adult Organs of Their Embryonic State—New Directions in Anti-Aging Regenerative Therapies

Authors: Klaudia Maar, Roland Hetenyi, Szabolcs Maar, Gabor Faskerti, Daniel Hanna, Balint Lippai, Aniko Takatsy, Ildiko Bock-Marquette

Source: https://pmc.ncbi.nlm.nih.gov/articles/PMC8228050/

Scientific Findings

This review discusses the role of Thymosin Beta-4 (TB4) in regenerative processes, particularly in the context of cardiac repair. The authors highlight their findings that external administration of TB4 promotes myocardial cell migration and survival in embryonic tissue in vitro. This effect is retained post-natally and is attributed to a direct effect on cardiac cells. The mechanism of action is partly explained by the activation of Integrin-Linked Kinase (ILK) and subsequent stimulation of Akt, which inhibits myocardial cell death. The review summarizes the growing body of evidence supporting the therapeutic potential of TB4 in cardiac regeneration and its potential to influence age-related changes in the heart and blood vessels.

Plain English Interpretation

This paper reviews the exciting potential of a naturally occurring molecule called Thymosin Beta-4 (TB4) to help our bodies heal and even reverse some of the effects of aging. The authors’ research has shown that TB4 can encourage heart cells to move and survive, which is crucial for repairing damage after a heart attack. They found that this happens because TB4 activates a specific chain of events inside the cells that prevents them from dying. This research suggests that TB4 could be a promising new treatment for heart disease and other age-related conditions, essentially ‘reminding’ our adult organs how to heal themselves like they did when we were embryos